The Anchiskhati Basilica of St Mary (Georgian: ანჩისხატი) is the oldest surviving church in Tbilisi, Georgia. It belongs to the Georgian Orthodox Church and dates from the sixth century.
According to the old Georgian annals, the church was built by the King Dachi of Iberia (circa 522-534) who had made Tbilisi his capital. Originally dedicated to the Virgin Mary, it was renamed Anchiskhati (i.e., icon of Ancha) in 1675 when the treasured icon of the Savior created by the twelfth-century goldsmith Beka Opizari at the Ancha monastery in Klarjeti (in what is now part of northeast Turkey) was moved to Tbilisi so preserve it from an Ottoman invasion. The icon was preserved at the Basilica of St Mary for centuries (it is now on display at the Art Museum of Georgia).